What are Jordi HPLC SEC GPC columns used for?

Jordi SEC/GPC columns are used for size exclusion chromatography (SEC) / gel permeation chromatography (GPC) to determine polymer molecular weight distribution, characterize biopolymers, and support QC/lot release testing.

Which Jordi column series are popular for GPC in organic solvents?

Jordi Resolve™ DVB and Jordi Gel DVB series are commonly selected for high-resolution GPC/SEC in organic solvents such as THF, depending on sample and molecular weight range.

Do you supply Jordi columns in India?

Yes. Akira Analytical Solutions Pvt Ltd supplies Jordi HPLC SEC GPC columns in India and can support selection, lead time, and quotations.

What is the difference between SEC and GPC?

SEC (size exclusion chromatography) is the general technique; GPC is commonly used to refer to SEC for polymers in organic solvents. Both separate molecules by hydrodynamic size.

Which solvent is commonly used for polymer GPC?

THF is a common solvent for many polymers (e.g., polystyrene). Depending on polymer chemistry, methods may also use DMF, HFIP, or aqueous buffers.

How do I select pore size for polymer SEC/GPC?

Choose pore size based on expected molecular weight range. If MW is unknown, mixed-bed columns can provide broad coverage; then optimize for resolution once the range is known.

Polymer SEC/GPC Applications with Jordi HPLC Columns

Learn how Jordi HPLC SEC GPC Columns are used for polymer molecular weight distribution, QC release testing, and characterization in common solvents such as THF.

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Typical SEC/GPC workflow

Select solvent/mobile phase: THF is common for polystyrene-type polymers; other methods may use DMF/HFIP or aqueous buffers.
Choose pore size / mixed-bed: based on expected molecular weight range (unknown samples often start with mixed-bed).
Detector selection: RI for universal detection; UV if chromophore is present; MALS/LS for absolute MW.
Calibration: use polymer standards where appropriate (e.g., PS standards in THF) or use absolute methods with LS.

Common questions (quick answers)

5 µm vs 13 µm particles

Smaller particles generally provide higher efficiency but can increase backpressure. Select based on instrument limits and target resolution.

Unknown polymer molecular weight

Start with a mixed-bed configuration that covers a broad MW range, then refine pore selection for best resolution.

Troubleshooting drift / noise

Check solvent degassing, detector equilibration, and sample filtration. Share your chromatogram and conditions for faster diagnosis.

Jordi SEC/GPC overview • Jordi Columns India

Need a recommendation?

Send these details: polymer type, solvent/mobile phase, expected MW range, detector, flow rate, and preferred dimensions. We will recommend the closest Jordi configuration and provide quotation/lead time.